Transcription Factor

Standard methods for studying transcription factor and gene regulation include gelshifts, western blots and reporter gene assays, which are time-consuming, do not allow for high-throughput and provide only semi-quantitative results. Alpha Innotech's Chemiluminescent FAST Transcription Factor Microplate Assays employ a proprietary assay technology and chemiluminescent detection that offers superior sensitivity, broad dynamic range and excellent reproducibility compared with traditional methods or with commercially available transcription factor ELISAs. The assays do not require the use of radioactivity, and results can be obtained in under four hours. The convenient stripwell format enables testing of from one to 96 samples in each plate. Chemiluminescence is used to measures the binding of activated the transcription factor to their cognate DNA binding sequences.