Alpha Innotech's FAST Transcription Factor Microplate Assay offers a chemiluminescent, 96 well microplate format for sensitive and specific detection of activated transcription factors. FAST is ideal for analyzing transcription factor activity in nuclear and cellular extracts prepared from cell lines, primary cells, and tissues. Kits are now available for a variety of important transcription factors in multiple species including human, primates and rat, with more now in development.

Advantages:

• Results in less than 4 hours
• High sensitivity
• No radioactivity, gels or antibodies
• Economical, convenient stripwell format
• Compatible with FluorChem Imaginf Systems and standard plate luminometers

Simple & Rapid Protocol

FAST Transcription Factor assay employs an elegent proprietary technology for detecting the presence of activated transcription factors in biological samples. The outcome: superior sensitivity, a broad dynamic range and excellent reproducibility compared with gel shift assays.

Assays currently available include:

• AP-1
• EGR
• NF-1
• NFkB
• PPAR
• Ask us about others in development!

Application Notes available for FAST Transcription Factor Microplate Assay:

Application Note 100: Detection of Active Transcription Factors in a 96 Well Plate Format (For FluorChem HD)