Guide
An EMSA is an electrophoretic mobility shift assay, also referred to as a gel shift assay, band shift assay, or gel retardation assay. EMSA is used to study protein-DNA interactions or protein-RNA interactions. Standard methods for studying transcription factors and gene regulation include EMSA, western blots and reporter gene assays, which are time-consuming, do not allow for high-throughput and provide only semi-quantitative results. Alpha Innotech's Chemiluminescent FAST Transcription Factor Microplate Assays employ a proprietary assay technology and chemiluminescent detection that offers superior sensitivity, broad dynamic range and excellent reproducibility compared with traditional methods. In addition, our FAST assays do not require the use of radioactivity and results can be obtained in under four hours, unlike time-consuming commercially available EMSA.
Standard methods for studying transcription factor and gene regulation include gelshifts, western blots and reporter gene assays, which are time-consuming, do not allow for high-throughput and provide only semi-quantitative results. Alpha Innotech's Chemiluminescent FAST Transcription Factor Microplate Assays employ a proprietary assay technology and chemiluminescent detection that offers superior sensitivity, broad dynamic range and excellent reproducibility compared with traditional methods or with commercially available transcription factor ELISAs. The assays do not require the use of radioactivity, and results can be obtained in under four hours. The convenient stripwell format enables testing of from one to 96 samples in each plate. Chemiluminescence is used to measures the binding of activated the transcription factor to their cognate DNA binding sequences.
Alpha Innotech's Fast Transcription factors kit offers a chemilumincent 96 well microplate format for sensitive and specific detection of activated transcription factors. Our kits measure activity from single or multiple nuclei or whole cell extracts prepared from cell lines, primary cells, and multiple tissue types. Activity can be measured in multiple species including human, rodent, and primates. This assay employs an innovative proprietary technology and chemiluminescent detection that can be used optimally on Alpha Innotech’s FluorChem ® Systems. The assays do not require the use of radioactivity. If you are a researcher working on transcription factors, we highly recommend you use this product. You can get a greater value for your research dollars and save time in the process with our transcription factors kits.
Alpha Innotech's ChemiGlow® chemiluminescent substrate is the world's first chemiluminescent substrate optimized for the FluorChem Family of CCD digital imaging systems. ChemiGlow substrate provides unsurpassed performance with the highest sensitivity and remarkably fast detection capabilities. In addition, Alpha Innotech offers the FAST Transcription Factor kit, which employs a proprietary assay technology to use chemiluminescent detection of a transcription factor binding to its target. Our FAST kit has a 96 well Microplate format for sensitive and specific detection of activated transcription factors. The convenient stripwell format enables testing of one to 96 samples in each plate at a time to provide customization of assay design. A FluorChem® Imaging system or a standard 96-well plate luminometer can be used to measure the FAST chemiluminescent signal.
Alpha Innotech develops and manufactures in vivo imaging and detection systems for the life science research and drug discovery markets. Our goal is to combine in vivo imaging instruments, reagents and bioinformatics software to offer integrated modular technology platforms for functional genomics, proteomics and cell analysis markets. Because all researchers employ a variety of techniques and applications in their laboratories and projects, Alpha Innotech strives to develop multi-functional imaging platforms that facilitate both basic imaging applications and complex applications, such as in vivo imaging. Our modular approach at development and integration also allows researchers to incorporate additional imaging applications into their project as the complexity of the project progresses. Our capabilities for in vivo imaging pattern this modular concept.
A protein array is widely becoming the platform chosen by researchers for quantitative determination of serum levels. A protein array can be spotted with target peptides, antibodies or complex mixtures utilizing protocols reminiscent of western blot and ELISA. Antibody microarrays exist in two general types: using matched antibody pairs for sandwich-type and relying on single printed antibodies and a direct sample labeling methodology. Cell and molecular biologists interested in measuring protein array levels can utilize standard slide formats or can scale up to 96 well plates to screen hundreds or thousands of samples. Some key requirements are multiplex detection utilizing multiple antibody tags, flexibility in adopting new dyes for specific antibody epitopes and high resolution imaging of high density spots with a protein array.
A gel shift assay, also referred to as an EMSA is an electrophoretic mobility assay. Gel shift is used to study protein-DNA interactions or protein-RNA interactions. Standard methods for studying transcription factors and gene regulation include gel shift, western blots and reporter gene assays, which are time-consuming, do not allow for high-throughput and provide only semi-quantitative results. Alpha Innotech's Chemiluminescent FAST Transcription Factor Microplate Assays employ a proprietary technology and chemiluminescent detection that offers superior sensitivity, broad dynamic range and excellent reproducibility compared with traditional methods or with commercially available transcription factor EMSA. Our FAST assays do not require radioactivity and results can be obtained in under four hours, which is a great improvement compared to traditional gel shift assays.
An AlphaImager or AlphaImager HP gel documentation system is used to detect, archive, and analyze fluorescent, and visible samples such as DNA, proteins and bacterial colonies. We also offer a full range of analysis software, from our easy to use AlphaEase FC to an advanced 21 CFR Part 11 compliant software package. If a customer’s needs change and they need more than a gel documentation system, they will be able to upgrade their system to a FluorChem family imager, allowing for the detection of chemiluminescent samples. All of our gel documentation systems provide easy to use real time sample positioning with a compact space requirement set at an affordable cost that fits most laboratory gel documentation system budgets.
The Fuji gel imaging systems are outperformed by our most innovative system, the Alpha Innotech FluorChem®HD2The FluorChem HD2 offers real time 16-bit documentation and an advanced combination of resolution, sensitivity and dynamic range. If you are evaluating fuji gel imaging systems, you should also consider the chemiluminescent, fluorescent, and colormetric applications possible with the FluorChem HD2. You will want to use the real time FluorChem HD2 for faster sample positioning, focusing and faster overall image capture than the fuji gel imaging systems. The goal of Alpha Innotech is to be a total solutions provider by bringing easy to use and cost effective solutions to the broader scientific community, including those currently using fuji gel imaging systems.
Considering a BioRad gel imaging system? You should also evaluate the FluorChem family of systems from Alpha Innotech.The FluorChem HD2 system from Alpha Innotech can outperform any BioRad gel imaging platform. For example, the VersaDoc MP 5000 BioRad gel imaging system only offers a resolution of 0.26 MP while our FluorChem HD2 provides a superior 4 MP resolution. Additionally, our FluorChem HD2 offers absolute and regulated cooling of the CCD detection chip to increase the signal to noise ratio during data acquisition. The VersaDoc system does not offer this feature. For enhanced resolution and the highest quality data during your image acquisition, please consider the Alpha Innotech FluorChem HD2 if you are currently evaluating BioRad gel imaging systems.
ChemiGlow substrate is the world's first western blot imaging chemiluminescence substrate optimized for CCD digital systems. This western blot imaging chemiluminescence substrate provides unsurpassed performance with the highest sensitivity. It has been optimized for superior performance by providing unequaled performance compared to film, which saves time and money by providing high dynamic range digital images at a fraction of the cost of film. It is available in flexible versions for all of your needs. ChemiGlow western blot imaging chemiluminescence substrate provides unsurpassed performance with the highest sensitivity. Transfer protein from the gel to a membrane. For best results, we recommend the use of nitrocellulose membrane. If you need a substrate that delivers an exceptional signal at an exceptional price use ChemiGlow western blot imaging chemiluminescence.
Our goal is to be a total electrophoresis imaging solutions provider by bringing easy to use and cost effective solutions in electrophoresis imaging, gel documentation, and chemiluminescent imaging to the broader scientific community. Flexibility in all of the Alpha Innotech platforms allows us to address multiple applications in the emerging molecular biology, proteomics, and systems biology marketplace. Our electrophoresis imaging systems for gel documentation are used to document, detect, archive, and analyze fluorescent, chemiluminescent and visible samples such as DNA, proteins and bacterial colonies. In addition, we offer a full range of software and consumable reagents to complement our detection systems, including a 21 CFR Part 11 compliant software package, as well as electrophoresis imaging platform and reagents.
Multicolor westerns are an ideal way to simultaneously detect multiple proteins closely related in size. Likewise, multicolor westerns allow evaluation of the phosphorylated and/or unphosphorylated state of proteins. Using antibodies with fluorophore conjugates, such as Quantum dots (Qdots), both sets of antibodies can be detected. The Alpha Innotech FluorChem family of imaging platforms integrates multiple filters into the emission filter wheel included in each darkroom component of the system. These filters allow researchers to target specific fluorophores, like those commonly used in multicolor westerns. Each fluorophore can be detected individually in a TIF file, and image files can be overlayed to present the detection of more than one fluorophore per sample. The FluorChem family of images is an ideal system to detect multicolor westerns.
Alpha Innotech develops gel analysis software for pharmaceutical and biotechnology companies as well as universities, medical centers, government research institutes and agencies worldwide. Founded in 1992, Alpha Innotech manufactures and markets digital imaging and detection systems as well as gel analysis software for the Life Science research and Drug Discovery markets. Our goal is to combine instruments, reagents and gel analysis software to offer integrated modular technology platforms for functional genomics, proteomics and cellomics markets. Alpha Innotech has over 30 thousands users of our products worldwide. Our products are sold in 35 plus countries. A key focus for Alpha Innotech is creating products with unparalleled ease-of-use, while providing a powerful array of features and flexibility suitable for a variety of gel analysis software applications.
ChemiGlow is a chemiluminescent substrate specifically designed for use with CCD cameras, such as the Alpha Innotech FluorChem family of imaging platforms. While most commericially available options for a chemilumincescent substrate produce short bursts of light and then fade quickly, ChemiGlow products a “glow” reaction which lasts for a longer period of time. The longevity of this reaction provides the researcher with added flexibility for signal detection, as well as the ability to collect as much signal as possible. Selection of an appropriate chemiluminescent subtrate is essential for optimal performance of your FluorChem Family imaging system. Alpha Innotech has a strong intellectual property portfolio in digital imaging, signal detection and micro array imaging as well as offering exceptional solutions for chemiluminescent substrate needs.
Alpha Innotech develops gel documentation systems for ethidium bromide imaging, as well as other fluorescent dyes for DNA or protein detection. Our products support research in the life sciences, and drug discovery markets. Alpha Innotech has over 30 thousand users of our ethidium bromide imaging products worldwide. Our ethidium bromide imaging products, such as the AlphaImager and AlphaImager HP are sold in 35 plus countries. Our customers include pharmaceutical and biotechnology companies as well as universities, medical centers, government research institutes and agencies worldwide. Our goal is to be a total solutions provider by bringing easy to use and cost effective solutions in gel documentation, array systems and ethidium bromide imaging to the broader scientific community.
Alpha Innotech’s FluorChem family of imagers are ideal western blot detection systems The FluorChem HD2 is a 4 MP, 16 bit imaging system with fast lens technology for optimal chemiluminescent detection. Western blot detection is made easy through AlphaEase FC software, which allows you to focus, zoom, and preview your image in real time. In addition to western blot detection, our user-friendly AlphaEase FC software provides users with both a documentation and analysis set of features. The FluorChem HD2, like our other members of the FluorChem family, is a perfect solution for single-user or multi-user facilities. Founded in 1992, Alpha Innotech Corporation develops, manufactures, and markets for the Life Science research and Drug Discovery markets digital imaging and western blot detection systems.
Our FluorChem Family of western blot imaging systems are used to document, detect, archive, and analyze fluorescent, chemiluminescent and bright field samples such as DNA, proteins and bacterial colonies. In addition, we offer a full range of analysis software to complement our documentation and detection systems including an excellent 21 CFR Part 11 compliant software package. Our new western blot imaging system, the FluorChem HD2, is our most innovative system to date for the detection of chemiluminescent signals and western blot imaging applications, as well as fluorescent and visible light applications. This flexible gel documentation and chemiluminescent detection platform offers Real Time 16-bit feedback and can be used for a number of applications, including western blot imaging applications.
If you are considering the Vilber Lourmat Chemi-Smart systems, you should also evaluate the Alpha Innotech FluorChem HD2. The FluorChem HD2 offers an exceptional 4MP resolution while the Vilber Lourmat Chemi-Smart only provides 1.3MP resolution. The FluorChem HD2 is cooled to -25 C and this cooling is absolute and regulated for the highest quality data, while the cooling of the Vilber Lourmat Chemi-Smart system is non-regulated, which allows fluctuations in temperature of the CCD chip and can lead to a less desirable signal-to-noise ratio. Additionally, the FluorChem HD2 offers a superior 16-bit dynamic range to capture both weak and robust signals within the same sample, while the Vilber Lourmat Chemi-Smart only offers a 12-bit dynamic range.
The AlphaImager family offers solutions for your 1 d gel imaging applications and needs. Both the AlphaImager and AlphaImager HP from Alpha Innotech provide real time sample positioning for easy image acquisition of your 1 d gel imaging samples. In addition, both systems within the AlphaImager family provide analysis quality data for both documentation and quantification of your data in a 1 d gel imaging format. The AlphaEase Analysis Software, a component of each of our systems, also provides solutions for detection, documentation, image enhancement, and analysis needs. Whether your project includes standard ethidium bromide detection or a more advanced detection method, Alpha Innotech offers a solution for your variety of 1 d gel imaging application
If your laboratory requires a fluorescence imager, evaluate the Alpha Innotech family of imaging systems. Both our AlphaImager and FluorChem family of systems include UV illumination options for both transparent and opaque samples. In addition, our systems include a 5 position emission filter wheel to accommodate a wide range of detection fluorophores. Our AlphaImager family provides a low-cost solution for researchers who need only the capabilities of a fluorescence imager, while our FluorChem family functions as both a fluorescence imager and a chemiluminescence detection system. Whether your applications needs are basic or advanced, Alpha Innotech offers a detection solution to meet your needs. Alpha Innotech strives to provide the most exceptional fluorescence imager solutions on the market today.
The needs of fluorescence imaging are met by the design of all of the Alpha Innotech systems. Both our AlphaImager and FluorChem family of systems include UV illumination options for both transparent and opaque samples. In addition, our systems include a 5 position emission filter wheel to allow researchers to target the detection of specific fluorophores of interest in their application. The integration of this filter wheel along with the filters offered by Alpha Innotech also accommodates a wide range of detection fluorophores and a variety of fluorescence imaging applications. Our AlphaImager family provides a low-cost solution for researchers focusing solely on fluorescence imaging, while our FluorChem family accommodates both fluorescence imaging and applications requiring chemiluminescence detection.
Requirements for 2 d gel imaging applications include enhanced sensitivity, spatial resolution, and dynamic range. The Alpha Innotech AlphaImager HP system provides a High Performance solution to meet these needs for 2 d gel imaging applications. The AlphaImager HPsystem offers a 1.4 million pixel resolution for exceptional spatial resolution to distinguish proteins in close proximity to each other on 2 d gel imaging samples. This high performance system also offers 12 bits of dynamic range to provide detection of both weak and very robust signals within the same sample while still avoiding saturation of the very strong signals. In addition to providing image acquisition solutions, Alpha Innotech also offers GelFox Analysis software to meet the analysis needs of 2 d gel imaging applications.
The AlphaImager gel doc system from Alpha Innotech has been created to provide easy to use real time sample positioning with robust analysis quality data at an affordable price that fits most laboratory budgets. Our AlphaImager gel doc and analysis system has been paired with our powerful AlphaEaseFC Imaging Analysis software that combines unrivaled ease of use with a comprehensive set of gel doc, analysis and image enhancement tools. This system provides a complete solution for the detection, documentation, and analysis needs of your laboratory. The AlphaImager system was developed in response to feedback from thousands of our valued Alpha Innotech customers who realize the importance of a robust, yet cost effective gel doc solution.
If your laboratory requires a solution for blot imaging applications, evaluate our FluorChem family of systems. Because blot imaging applications present challenges for resolution, sensitivity, and dynamic range, Alpha Innotech has designed an exceptional range of systems that provide quantifiable data for multiple blot imaging applications. Our FluorChem HD2 system offers the only 16-bit CCD camera with a real time refresh rate, which facilitates easy sample positioning and focusing. This system also provides a 4 million pixel CCD chip, which translates into exceptional image resolution and provides sharp images even if your features of interest are in close proximity. Whether your application needs utilize chemiluminescent or fluorescent detection methods, Alpha Innotech provides an appropriate blot imaging solution for you.
If chemiluminescence is the major application in your laboratory and you are seeking the absolute best in sensitivity, then the Alpha Innotech FluorChem HD2 is the ideal product for you and your team. The FluorChem HD2 is our most sensitive chemiluminescence imaging system available. It is versatile enough to handle all the varied imaging needs of your laboratory, but sensitive enough for your most challenging samples. The FluorChem HD2 is a 16-bit, 4 million pixel system, which provides exceptional dynamic range for capturing both weak and very strong chemiluminescence signals within the same sample. When used with our ChemiGlow reagent kit, the FluorChem HD2 will give you the best sensitivity available for your chemiluminescence samples.
If your research requires a solution for multiplex imaging applications, evaluate the Alpha Innotech FluorChem family of systems. All of our FluorChem systems offer fluorescent and bright field options for multiplex imaging applications by providing both UV and white light illumination sources. In addition, a 5 position emission filter wheel expands capabilities for multiplex imaging options by allowing researchers to target the detection of specific fluorophores of interest.The AlphaEase Analysis software included with each system also provides a comprehensive set of documentation, image enhancement, and analysis solutions. Alpha Innotech has designed its FluorChem Family of systems with key advantages in resolution, sensitivity, dynamic range and performance to meet the growing needs of multiplex imaging applications.
Alpha Innotech provides multiple microarray reader solutions. For researchers needing a robust laser-based microarray reader, our AlphaScan dual-laser system provides reliable and accurate data. It also accommodates a wide variety of slide-based substrates. For researchers requiring enhanced flexibility in a microarray reader, our NovaRay imaging system provides flexibility in detection fluorophore choice by accommodating the detection of up to 8 different fluorophores, as well as allowing versatility in substrate options by accommodating both slide-based and 96-well plate-based assays. The NovaRay maintains the ease of use necessary for either a single laboratory or a multi-user core facility while still providing the assay flexibility desired for a progression in project complexity. Both of these solutions offer the impressive data quality necessary for your choice of microarray reader.
Protein arrays are widely becoming the assay platform most chosen by researchers for quantitative determination of protein levels. This assay design increases throughput and preserves sample quantity. Protein arrays can be created by immobilizing target peptides, antibodies or complex mixtures in solution onto a variety of substrates. These substrates can include slides and microwell plates with specially treated surfaces to enhance protein binding. Antibody protein arrays can be composed of matched antibody pairs for sandwich-type assays or single antibody immobilization for the capture of directly-labeled samples. Our NovaRay imaging system accommodates both a slide-based and mutiwell microplate-based format to provide advanced flexibility in assay design for cell and molecular biologists with an interest in protein arrays.
Alpha Innotech’s AlphaScan™ is a compact and high performance microarray scanner made for imaging and data analysis of DNA, protein, cell and tissue arrays. This microarray scanner integrates advanced designs in its optical, signal processing, and motion control systems resulting in superior performance capabilities when compared to similar microarray scanner products. With two excitation lasers with wavelengths of 532 and 635nm, AlphaScan™ provides high sensitivity, accuracy, dynamic range and linearity, thus offering an ideal tool for gene expression profiling of arrays. Our NovaRay Detection Platform also provides unparalleled flexibility for applications in functional biology, cellomics, gene expression and drug discovery research. Alpha Innotech offers some of the highest quality, most efficient, cost effective microarray scanner systems on the market today.
Alpha Innotech provides two solutions for microarray applications with expression arrays. The NovaRay addresses the growing microarray applications market by accommodating a multiplexed technology platform in both an array-of-arrays format in microplates, as well as other slide-based substrates. The AlphaScan accommodates those evaluating applications, including expression arrays, on more traditional slide-based substrates. Because this application area requires the acquisition of reproducible and accurate data, both our NovaRay and AlphaScan system provide the robust application utility and versatility needed to address your project goals. These two microarray imaging platforms provide flexibility for a variety of applications with expression arrays. Alpha Innotech provides a microarray imaging solution for your research needs whether you are evaluating CGH arrays, antibody microarray assays, or expression arrays.
Alpha Innotech develops digital gel imaging and analysis systems for documentation, quantitative analysis, gene expression studies, and a wide range of other Life Science applications. Alpha Innotech maintains a staff of software, mechanical, and optical engineers, as well as a team of in-house and field-based application specialists who assist not only our gel imaging customers, but also our users of more advanced documentation and analysis platforms. Our Technical Support Department provides our customers with telephone and email support for all of our gel imaging products. We strive to provide our worldwide users, including users in more than 35 countries, with the level of support expected and deserved. Alpha Innotech also supports a wide range of application needs; include those of our gel imaging customers.
At Alpha Innotech, we develop digital gel imager and analysis tools for documentation, quantitative analysis, gene expression studies, and a wide range of other Life Science applications. Our customers include the biotechnology, pharmaceutical, and university sectors in more than 35 countries. To support this worldwide user base Alpha Innotech maintains a comprehensive staff of software, mechanical, and optical engineers as well as a team of in-house and field-based application specialists to assist all of our gel imager product users. Our Technical Support Department provides our customers with telephone and email support for our gel imager product family. Alpha Innotech also provides a wide range of other imaging platforms if your applications require more than a gel imager.
If you are in the market for a Fuji gel imager, such as the LAS-3000, it would benefit you to take a look at our FluorChem family of imaging systems. As an overview, the FluorChem HD2 is a versatile and sensitive, real time, 16-bit, 4 million pixel cooled CCD imaging system while the Fuji gel imager (LAS-3000) is a 16-bit, 3.15 million pixel cooled, slower refresh imaging system. The Fuji gel imager requires the use of 2 software packages to run while the FlourChem HD2 only requires one. When looking for an imaging system that provides market leading resolution, sensitivity, dynamic range, and performance consider Alpha Innotech’s FluorChem family instead of a Fuji gel imager.
The AlphaImager HP is a popular gel imaging product and analysis system from Alpha Innotech. The AlphaImager HP is priced for individual users, while providing the data quality and analysis capabilities needed by a multi-user facility. This AlphaImager HP platform integrates ease of use with high performance to provide the user interface your lab expects and the data quality your research needs. Our AlphaImager HP gel imaging product and analysis system has been paired with our powerful AlphaEaseFC Analysis software that combines unrivaled ease of use with a comprehensive set of gel imaging product documentation, analysis and image enhancement tools. The AlphaImager HP serves as both an outstanding gel imaging product and an excellent data analysis system.
The Alpha Imager gel documentation system from Alpha Innotech has been created to provide easy to use real time sample positioning, image acquisition and analysis quality data at an affordable price that fits most laboratory budgets. Our Alpha Imager gel documentation and analysis system has been paired with our powerful AlphaEaseFC Imaging Analysis software that combines unrivaled ease of use with a comprehensive set of gel documentation, annotation, analysis, and image enhancement tools. The Alpha Imager also serves as a multi-functional imaging system for increased application flexibility and versatility. Feedback from thousands of our valued Alpha Innotech customers was gathered and utilized to help us develop this high quality Alpha Imager gel documentation and analysis system.
